Extended Data Fig. 1: Dynamic redundancy by HGT promoted the gene abundance stability of two-strain communities transferring a single plasmid.
From: Horizontal gene transfer enables programmable gene stability in synthetic microbiota
a. The conjugation efficiency of R388 between MG1655 and Top10. Data are presented as mean values +/− standard deviations of three replicates. b. The growth curves of MG1655 and Top10 under different Strp concentrations. Data are presented as mean values +/− standard deviations of three replicates. c. The maximum growth rates of MG1655 and Top10 under different Strp concentrations. Data are presented as mean values +/− standard deviations of three replicates. d. The fitness burden of R388 in MG1655 or Top10. Data are presented as mean values +/− standard deviations of three replicates. e. Linoleic acid (LAC) inhibited the transfer of R388. The conjugations rates were normalized with the mean rate without LAC treatment. Data are presented as mean values +/− standard deviations of three replicates. f. Streptomycin did not impact the inhibition effects of linoleic acid on R388 transfer. The conjugations rates were normalized with the mean rate of the control group (0 mM LAC and 0 μg/mL Strp). Data are presented as mean values +/− standard deviations of three replicates. g. The temporal dynamics of Top10 relative abundances in the five communities during the experiment. Data are presented as mean values +/− standard deviations of three replicates. h. The relationship between community composition and R388 abundance at day 15. Data are presented as mean values of three replicates. i. The gene abundance stability ϕ increases with the plasmid transfer rate in a model of two species transferring a single plasmid. The x-axis is divided into multiple bins with widths of 0.015. Error bars represent mean +/− standard deviation of the ϕ values in each bin (n = 200 independent data points).
