Extended Data Fig. 3: A two-species model predicts the plasmid R388 dynamics in monocultures or cocultures of MG1655 and Top10.
From: Horizontal gene transfer enables programmable gene stability in synthetic microbiota
Dynamic parameters, including the plasmid transfer rates, strain growth rates under different Strp doses, plasmid burdens and LAC inhibition effects were measured and used to parameterize the model. a. Dynamics of R388 abundances in MG1655 or Top10 cultured separately. For each strain, three LAC doses and two dilution ratios were tested. Experiment data are presented as mean values +/− standard deviations of three replicates. b. Dynamics of R388 abundances and strain compositions in cocultures of MG1655 or Top10. Five communities, treated by different concentrations of Strp, were modeled or experimentally measured. For each community, three LAC doses and two dilution ratios were tested. Experiment data are presented as mean values +/− standard deviations of three replicates. c. The degree of gene abundance stability in the MG1655-Top10 cocultures increased with the plasmid transfer rate. The results of day 5, 10 and 15 were shown in different line styles, while the two dilution ratios were represented by different marker styles.
