Extended Data Fig. 6: PITCOIN1 and PITCOIN3 do not affect cellular PI(4,5)P2 and PI(4)P levels.
From: Development of selective inhibitors of phosphatidylinositol 3-kinase C2α
(a) Representative confocal microscopy images of fixed Cos7 cells treated with DMSO, PITCOIN1, or PITCOIN3 (20 μM for 6 h) and stained for PI(4,5)P2 using specific antibodies. (b) Quantification of representative data shown in (a). Data are from n = 3 independent experiments and depicted as mean ± SEM. N.S., non-significant, one-way ANOVA with Tukey’s multiple comparison test. (c) Representative confocal microscopy images of fixed Cos7 cells treated with DMSO, PITCOIN1, or PITCOIN3 (20 μM for 6 h) and stained for PI(4)P on the cell surface using specific antibodies. (d) Quantification of representative data shown in (c). Data are from n = 3 independent experiments and depicted as mean ± SEM. N.S., non-significant, one-way ANOVA with Tukey’s multiple comparison test. (e) Representative confocal microscopy images of fixed Cos7 cells treated with DMSO, PITCOIN1, or PITCOIN3 (20 μM for 6 h) and stained for internal pools of PI(4)P using specific antibodies. Cells treated with 5 µM PI4KIIIβ-IN-10 for 2 h were used as a positive control. (f) Quantification of representative data shown in (e). Data are from n = 3 independent experiments and depicted as mean ± SEM. N.S., non-significant, one-way ANOVA with Tukey’s multiple comparison test.
