Extended Data Fig. 5: Assessing the susceptibility of EKO-35 harboring ASKA plasmids expressing rspA, tufA, pitA, wcaC, gyrB, and yjfC.
From: A genetic platform to investigate the functions of bacterial drug efflux pumps
Strains were profiled against a, benzalkonium chloride, b, novobiocin, c, trimethoprim, d, synthetic 2, e, synthetic 14, f, synthetic 19. MICs were determined i, without and ii, with chloramphenicol to maintain ASKA plasmids. Gene expression was induced with 0.1 mM IPTG. 25 µg/mL and 4 µg/mL chloramphenicol were used to maintain ASKA plasmids in wild-type E. coli and EKO-35, respectively. Strains were assessed in technical duplicate.
