Extended Data Fig. 7: Heat maps depicting susceptibility levels of strains expressing efflux pump-encoding genes.
From: A genetic platform to investigate the functions of bacterial drug efflux pumps
Each strain was tested in technical duplicate and MIC values were normalized to 100% for each compound tested, where orange represents the highest MIC value, and white represents the lowest value (see key). Genes encoding a, AcrEF, b, AcrD, c, AcrB, d, MdtEF, e, MdtBC, f, MacAB, g, MacAB, and h, MexCD were chromosomally integrated into the wild-type strain, a single gene deletion mutant, and EKO-35. Susceptibility testing revealed no changes in the resistance levels of the wild-type or single gene deletion backgrounds, except for c, ΔacrB. EKO-35 was highly susceptible to all compounds tested, revealing susceptibility profiles similar to ΔtolC, and integration of efflux genes into this strain conferred resistance to the known substrates.
