Extended Data Fig. 8: Heat maps depicting aminoglycoside susceptibility of the wild-type K-12 strain and an ΔacrD mutant expressing the chromosomally integrated acrD gene (araC::acrD).
From: A genetic platform to investigate the functions of bacterial drug efflux pumps
Susceptibility testing was performed in both MHB and LB. a, Kanamycin in cation-adjusted Mueller Hinton II Broth (MHB II), b, Kanamycin in Lysogeny broth (LB), c, Gentamicin in MHB II, and d, Gentamicin in LB. The cell inoculum used was 104 cells/mL to replicate susceptibility testing conditions used in previous studies. Each strain was assessed using three technical replicates and MIC values were normalized to 100% for each compound tested, where orange represents the highest MIC value, and white represents the lowest value (see key).
