Extended Data Fig. 10: EKO-35 and the efflux platform can be used to assess efflux pump interplay.
From: A genetic platform to investigate the functions of bacterial drug efflux pumps
Interplay was not observed for novobiocin in a, EKO-35 and b, EKO-35-Pore or for minocycline in c, EKO-35 and d, EKO-35-Pore. pGDP-2 harboring emrE is denoted as pEmrE. All integrated strains were transformed with the empty vector (pGDP-2). Genes encoding AcrB, AcrEF, AcrD, and MdtEF were integrated into the arabinose operon (araC) of EKO-35. Related to Fig. 5 and Supplementary Dataset 5. Data points represent mean MIC values for which the OD600nm value >0.100 ± s.d. of n = 3 biological replicates. P-values were calculated by a two-tailed Student’s t-test (ns = not significant).
