Extended Data Fig. 3: Plasmid-based complementation of genes in EKO-35 harboring genomic mutations alters biofilm formation in EKO-35 and wild-type E. coli.
From: A genetic platform to investigate the functions of bacterial drug efflux pumps
Expression of rspA significantly increased biofilm formation in a, wild-type E. coli (P = 2.67 × 10−3) and b, EKO-35 (P = 3.35 × 10−2) in Lysogeny broth, whilst expression of pitA, tufA, gyrB, and yjfC significantly lowered biofilm formation in wild-type E. coli (P = 4.70 × 10−4, 2.98 × 10−5, 6.56 × 10−3, 1.09 × 10−3 respectively) and pitA, tufA, and yjfC in EKO-35 (P = 3.00 × 10−3, 1.65 × 10−1, 8.36 × 10−2, 3.09 × 10−3 respectively). Gene expression was induced using 0.1 mM IPTG and plasmid selection for EKO-35 was achieved using 1 µg/mL chloramphenicol in M9 minimal glucose medium and 4 µg/mL in Lysogeny broth. Data points represent mean values ± s.d. of n = 3 biological end-point readings after 24 h and 48 h of growth in nutrient-rich and -limited media, respectively. P-values were calculated by a two-tailed Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant).
