Extended Data Fig. 5: INSPIRE-mediated activation of a reporter gene.
From: Chemically inducible split protein regulators for mammalian cells
a, Schematic presentation of the transcriptional activator INSPIRE systems (TA-INSPIRE). Ligand-induced dimerization of split protein fragments results in the expression of firefly luciferase gene (fLuc) on a reporter plasmid with 10 binding sites for gRNA upstream of the minimal promoter (Pmin). b–g, Transcriptional activation of the fLuc reporter gene by employing LYN267- (b), DHFR173- (c), GR2175- (d), TRβ412- €, ERβ446- (f), and PPARγ429- (g) based TA-INSPIRE systems after stimulation of HEK293T cells with the indicated ligands. Results are shown as fold activation in reporter gene expression compared to non-stimulated mock cells transfected with reporter plasmid only. Detailed descriptions of the genetic components and transfection mixtures are provided in Supplementary Table 4. Plots shows the means ± s.d. of n = 8 biological replicates combined from two independent experiments. See Supplementary Table 8 for sample sizes (n) and P values. Source data are provided as a Source Data file.
