Extended Data Fig. 6: INSPIRE-mediated activation of individual endogenous genes.
From: Chemically inducible split protein regulators for mammalian cells
a,b, Activation of individual endogenous genes by employing GR2- (a) or ERβ- (b) based TA-INSPIRE systems. HEK293T cells were transfected with individual gRNAs targeting TTN, ASCL1, NEUROD1, and TUNAR endogenous genes and stimulated with indicated ligands. Results are shown as fold activation in reporter gene expression compared to non-stimulated mock cells transfected with an empty plasmid (pcDNA3.1). Detailed descriptions of the genetic components and transfection mixtures are provided in Supplementary Table 4. Plots shows the means ± s.d. of n ≤ 6 biological replicates combined from two independent experiments. Conditions were compared using a two-sided unpaired t-test with Welch correction (****P < 0.0001; ***P < 0.001; **P < 0.01; ns > 0.05). See Supplementary Table 8 for sample sizes (n) and P values. Source data are provided as a Source Data file.
