Extended Data Fig. 10: Evaluation of GR2-INSPIRE responsiveness to corticosterone (CORT) and prescreen of the effective gRNAs targeting the mouse 11β-HSD2 gene.
From: Chemically inducible split protein regulators for mammalian cells
a, The dose-response curve of GR2175-based INSPIRE system for corticosterone (CORT), utilizing dual luciferase assay in HEK293T cells. b, Activation of the mouse 11β-HSD2 gene in Neuro2a cells transfected with dCas9-VPR and indicated gRNAs. The light shaded region indicates the gRNA with the highest fold activation. Results are shown as fold activation in endogenous gene expression compared to mock cells transfected with an empty plasmid (pcDNA3). Detailed descriptions of the genetic components and transfection mixtures are provided in Supplementary Table 4. Plots shows the means ± s.d. of n = 6 biological replicates combined from two independent experiments. Conditions were compared using a two-sided unpaired t-test with Welch correction (****P < 0.0001; **P < 0.01; ns > 0.05). See Supplementary Table 8 for sample sizes (n) and P values. Source data are provided as a Source Data file.
