Extended Data Fig. 8: Lack of S-nitrosylation of Spike protein and E protein.
From: Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection
a, Purified recombinant SARS-CoV-2 Spike (S1 + S2) protein and ACE2 protein were exposed to 100 μM SNOC; 30 min later, samples were subjected to biotin-switch assay in the presence or absence of ascorbate (Asc) to assess protein S-nitrosylation. n = 3 biological replicates. b, Lack of E protein S-nitrosylation by NMT5. HA-tagged E protein plasmid was transiently transfected into HEK293 cells. One day after transfection, cells were exposed to 10 μM NMT5. After 1 hour, the cells were harvested and subjected to biotin-switch assay in the presence or absence of ascorbate (Asc) to assess protein S-nitrosylation, which was detected by immunoblotting with anti-HA antibody. n = 3 biological replicates.
