Extended Data Fig. 1: DHT-stimulated androgen receptor formed phase-separated condensates.
From: Targeting androgen receptor phase separation to overcome antiandrogen resistance
a. Immunofluorescence (IF) of AR in VCaP cells (AR amplification and some V7). Fluorescence signal is shown alone (green), merged with DAPI stain (blue) (Scale bar, 5 μm) and zoomed view of merged image (Scale bar, 350 nm). b. Live imaging of mEGFP tagged AR in VCaP cells. mEGFP signal is shown alone (green), mEGFP merged with Hoechst stain (blue). Scale bar, 5 μm. c. The immunoblot analysis of AR proteins in LNCaP and VCaP cells stably expressed with AR–mEGFP. d. Live imaging of mEGFP tagged AR in PC3 cells. mEGFP signal is shown alone (green), mEGFP merged with Hoechst stain (blue). Scale bar, 5 μm. e. Live imaging of mEGFP tagged AR in HEK293T cells. mEGFP signal is shown alone (green), mEGFP merged with Hoechst stain (blue). Scale bar, 5 μm. f. Live imaging of mEGFP tagged AR in H1299 cells. mEGFP signal is shown alone (green), mEGFP merged with Hoechst stain (right). Scale bar, 5 μm. g. Live imaging of mEGFP tagged AR in SF268 cells. mEGFP signal is shown alone (green), mEGFP merged with Hoechst stain (blue). Scale bar, 5 μm. h. Live imaging of mScarlet tagged AR in LNCaP cells. mScarlet signal is shown alone (magenta), mScarlet merged with Hoechst stain (blue). Scale bar, 5 μm. i. Schematic and sequencing validation of mEGFP knock-in strategy. j. Representative images of endogenous tagged AR–mEGFP treated with/without DHT stimulation. Scale bar, 5 μm (zoomed, 1 μm).
