Extended Data Fig. 6: Cas9-independent off-target assessment in the R-loop assay.
From: Engineering a precise adenine base editor with minimal bystander editing
a, On-target base editing induced by ABE8e, ABE8e-N108Q or ABE9 using the modified orthogonal R-loop assay at each R-loop site with nSaCas9-sgRNA plasmid. Data are mean ± s.d. (n = 3 independent experiments). b, Comparison of indels induced by ABE8e, ABE8e-N108Q or ABE9 at six R-loop sites from Fig. 4b. Each data point represents the average indel frequency at each target site calculated from 3 independent experiments. Error bar and P value are derived from these 6 data points. Data are mean ± s.d. P value was determined by two-tailed Student’s t test. Statistical source data are provided online.
