Extended Data Fig. 7: Highly efficient and precise editing by ABE9 in rodent embryos.
From: Engineering a precise adenine base editor with minimal bystander editing
a, Genotyping of F0 generation pups treated by ABE8e or ABE9. b, Comparison of indels induced ABE8e (n = 19) or ABE9 (n = 16) in the target sequence of the splicing acceptor site in intron 3 of the mouse Tyr gene. Data are mean ± s.d. c, Summary of the numbers of embryos used and the pups generated after microinjection of ABE8e/sgRNA or ABE9/sgRNA. d, e, Genotyping of F0 rats induced by ABE7.10 (d) and ABE9 (e) (desired editing in blue or undesired in red). f, Comparison of indels induced by ABE7.10 (n = 28) or ABE9 (n = 8) in the target sequence of exon 13 in the rat Gaa gene. Data are mean ± s.d. g, Ratio of desired reads to total reads in F0 rats induced by ABE7.10 or ABE9. In a, d and e, the percentage on the right represents the frequency determined by the rate of indicated mutant alleles to total alleles counts. Percentiles of each allele reads <1% are omitted. Statistical source data are provided online.
