Extended Data Fig. 1: ABE8e induces severe bystander mutations and global random off-target editing.
From: Engineering a precise adenine base editor with minimal bystander editing
a, Comparison of base editing efficiency of ABEmax or ABE8e at 6 endogenous genomic loci in HEK293T cells. Partial data are derived from Fig. 1b, c. b, The C-to-T/G/A editing efficiency of ABEmax or ABE8e was examined at 5 endogenous genomic loci in HEK293T cells. Partial data are derived from Fig. 1c. c, The schematic diagram of orthogonal R-loop assay-based nickase SaCas9 (nSaCas9) (left panel); Cas9-independent DNA off-target analysis of ABE8e using the modified orthogonal R-loop assay at each R-loop site with nSaCas9-sgRNA plasmid (right panel). Data are mean ± s.d. (n = 3 independent experiments). Data are derived from Fig. 4b. In a and b, the heatmap represents average editing percentage derived from three independent experiments and editing efficiency was determined by HTS. Statistical source data are provided online.
