Extended Data Fig. 6: H3 peptide binding of purified PWWP domains.
From: Base editor scanning charts the DNMT3A activity landscape
Binding of purified PWWP domain variants to biotinylated H3K36me0 or H3K36me2 peptides (H3 residues 21–44). Bound proteins were captured by streptavidin pulldown, resolved by SDS–PAGE, and visualized by silver staining. This experiment was performed in two independent trials, and both are shown. Within each experimental trial, gels were electrophoresed and stained in parallel. A longer silver stain exposure was used for trial 1 than for trial 2. The PWWP truncation used here (residues 278–427, untagged, pI = 5.45 (Expasy ProtParam)) is negatively charged at the assay pH (pH = 7.5), which could promote nonspecific interactions with the positively charged histone peptide. Uncropped gels are provided as Source Data.
