Extended Data Fig. 6: Selectivity of compound-16 and ML382 among other GPCRs.
From: Ligand recognition and allosteric modulation of the human MRGPRX1 receptor
a-b, Screening of ML382 (a) and compound-16 (b) in single concentration (10 µM) at 318 receptors in PRESTO-Tango GPCRome assays. Dopamine (D2) with 100 nM quinpirole was used as the assay control (assay ctrl). The data are plotted as fold of basal activity and presented as mean ± SEM (n = 4). The ones with >3-fold of basal were took as pronounced hits. c-h, Follow-up dose response curves for the targets with > 3-fold increased activity. Known agonist for each receptor was used as positive controls, and all the results were normalized to these controls (c-g). For MRGPRX1, the results were plotted as fold of basal (g-h). All the data are presented as mean ± SEM of n = 3 independent experiments.
