Extended Data Fig. 6: Relative accumulation of CoA intermediates in the feedback systems with and without module 5.
From: Synthetic anaplerotic modules for the direct synthesis of complex molecules from CO2
All left panels (a) show relative module 1 intermediate accumulation over time without module 5 (DEBS), right panels show intermediate accumulation with module 5 (b). Addition of module 5 had a consistently suppressive effect on the accumulation of intermediates compared to the setups without. The highest detectable extracted ion count via HPLC-MS (as EIC peak areas) measurement of each individual CoA thioester in any assay shown in Fig. 4 was set to 11.11% (9 compounds = 100%). All other EIC peak areas of that compound were set relative to that to determine the relative abundance. For absolute values of the EIC peak areas see Supplementary Fig. 4. Setups containing only module 1 (± module 5) were started with propionyl-CoA (167 µM in 1, and 333 µM in 1.1), all others with 250 µM glyoxylate.
