Extended Data Fig. 4: 6-dEB quantification.
From: Synthetic anaplerotic modules for the direct synthesis of complex molecules from CO2
6-dEB formation was quantified by NADPH oxidation (6 NADPH molecules per 6-dEB molecule). This data was used to calibrate HPLC-MS measurements (EIC peak area). a Spectrophotometric NADPH oxidation of DEBS (in duplicates; green and orange) and a negative control without substrates (gray). b After establishing stoichiometric equivalence between NADPH oxidation and 6-dEB production, the reduction rates were correlated to the 6-dEB HPLC measurements in increments of 20 minutes, performed as technical duplicates. Note that the ordinate offset has been subtracted to yield the concentrations given in the main text.
