Extended Data Fig. 5: Extended version of Fig. 4a,b.
From: Synthetic anaplerotic modules for the direct synthesis of complex molecules from CO2
Setups containing anaplerotic sequences (1-2-3-4[a/b/c/d]) were started with 250 µM glyoxylate (C2). Module 1 alone was started with either an equivalent amount of propionyl-CoA (C3, 167 µM) or twice the amount (333 µM, labeled as 1.1). a Methylmalonyl-CoA accumulation in the different feedback pathways and both setups of module 1 alone. Note that the 0 h samples for both setup 1 and 1.1 contained detectable amounts of methylmalonyl-CoA due to a fast turnover immediately after addition of starting substrate (propionyl-CoA). b 6-dEB production by the different pathways and a control using only the isolated polyketide synthase. *not detectable **positive control was started with 0.8 mM propionyl-CoA and 1.0 mM methylmalonyl-CoA. All experiments were performed in technical duplicates and are displayed as mean (white squares) and their individual data points (colored squares).
