Extended Data Fig. 3: CARD8 inflammasome activation is dependent on Gag-Pol expression.

a, Representative flow cytometry plots of CARD8-KO or Cas9 control THP-1 cells treated with DMSO, EFV (3 µM), VbP (1 µM), or combination. Heatmap plots are colored according to mean fluorescent intensity (MFI) of GFP with higher MFI colored in red and lower MFI in green. b, Mean fluorescent intensity of three replicates from (a). Two-way ANOVA with Dunnett’s multiple comparison test. c, Frequency of HIV-1 DNA⁺ cells measured by qPCR. CARD8-KO or Cas9 control THP-1 cells were infected with HIV-1 for 3 days and treated with DMSO, EFV (3 µM), VbP (1 µM), or combination for 2 days. HIV-1 gag levels were normalized to POLR2A to determine the frequency of HIV-1 DNA⁺ cells. The frequency in the treatment groups was measured as the percent of the DMSO control (n = 3). Two-way ANOVA with Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01 and **** p < 0.0001. Error bars show mean values with SEM.