Extended Data Fig. 5: Plasmid maps and control experiments for planktonic-biofilm transition.
a, Map of the plasmid IS5-Zn-gfp-prob. b, Map of the plasmid P45-Zn-gfp. c, Gene circuit of the plasmid P45-Zn-gfp. d-i, State transition experiments for the strain carrying the plasmid P45-Zn-gfp under different temporal patterns of zinc availability. Compared to the case of the strain carrying the plasmid IS5-Zn-gfp-prob (Fig. 4), the biofilm of the P45-Zn-gfp loaded strain cannot be decomposed once it forms. Experimental data are presented as mean ± s.d. from 3 independent experiments.
