Extended Data Fig. 4: SEU condensation is sensitive to crowding.
From: Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis
a, Representative confocal microscopic images of yeast cells expressing IDR1-GFP. The cells were treated with or without NaCl and KCl for 5 min. Scale bars, 2 μm. b, Representative confocal microscopic images of yeast cells expressing SEU-IDR13KR-GFP. The cells were treated with or without sorbitol for 5 min. Scale bars, 2 μm. c, Representative confocal microscopic images of tobacco epidermal cells expressing SEU-mVenus under the ethanol inducible promoter. The cells were treated with 1% ethanol for indicated time. Scale bars, 5 μm. d, Coomassie staining of indicated protein samples before and after TEV cleavage to remove the MBP tag. e, In vitro phase separation assay of IDR1-mScarlet in the presence of indicated concentrations of PEG, NaCl or KCl. Scale bars, 5 μm.
