Extended Data Fig. 1: SEU undergoes condensation in response to hyperosmotic stress.
From: Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis
a, Illustration of the screen for osmotic stress-responsive biomolecular condensates. b, Representative confocal microscopic images of SEU-GFP expressed in yeast cells that were treated as indicated. Scale bars, 2 μm. c, Representative confocal microscopic images of tobacco epidermal cells expressing SEU-mVenus. The cells were treated as indicated. Scale bars, 5 μm. d, The protein levels of SEU and SEU-GFP in the indicated plants as determined by western blot. e, Expression of SEU relative to ACTIN in the indicated plants. Data are presented as the mean ± SD (n = 3 biologically independent experiments). Asterisk indicates a significant difference (two-tailed t test). f-i, Representative confocal microscopic images of Arabidopsis root tip cells expressing SEU-GFP. The roots were treated as indicated. Scale bars, 5 μm. j, Immunostaining of Arabidopsis root tip nuclei from plants treated with or without 300 mM NaCl. Scale bars, 5 μm. k, Representative confocal microscopic images of yeast cells expressing SEU. The cells were treated as indicated. Scale bars, 2 μm. l, Representative confocal microscopic images of tobacco epidermal cells expressing SEU. The cells were treated as indicated. Scale bars, 5 μm.
