Extended Data Fig. 2: Analyses of SEU-Like proteins (SLKs).
From: Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis
a, Multiple sequence alignment of SEU and SLK protein sequences by Clustal W. b, The phylogenetic tree of SEU and SLK proteins inferred using the Neighbor-Joining method. c, The protein domain structures of SLKs. Disordered regions were predicted by IUpred algorithm. d, Representative confocal microscopic images of SLK2-GFP expressed in yeast cells that were treated by sorbitol for 30 min. Scale bars, 2 μm. e, The protein domain structure of a chimeric protein swapping the IDR1 of SLK2 with that of SEU. f, Representative confocal microscopic images of tobacco epidermal cells expressing the chimeric protein shown in (e). The cells were treated with or without NaCl for 5 min. Scale bars, 5 μm unless indicated.
