Extended Data Fig. 7: Single clone analysis of depleted cluster variants.
From: Deep mutational scan of a drug efflux pump reveals its structure–function landscape
Growth of L. lactis NZ9000 ΔlmrCD ΔlmrA expressing the indicated depleted cluster variants in the presence of daunorubicin (red, 8 µM), ethidium (green, 16 µM) or Hoechst (blue, 1.5 µM) is shown as straight line. Cells expressing wild- type EfrCD (wtEfrCD, dashed line) or the inactive E512QEfrD variant (dotted line) were included as controls. The growth experiment was carried out on three separate days, resulting in three biological replicates.
