Extended Data Fig. 1: Characterization of nanobody Nb_EfrCD#1.
From: Deep mutational scan of a drug efflux pump reveals its structure–function landscape
a, Cartoon representation of Nb_EfrCD#1 binding to the extracellular part of EfrCD. Complementary determining regions (CDRs) 1, 2 and 3 are colored in yellow, orange and red, respectively. b, Affinity determination of Nb_EfrCD#1 using grating-coupled interferometry (GCI). EfrCD was immobilized on a WAVEchip and Nb_EfrCD#1 was injected at 0.33, 1, 3, 9 and 27 nM. A 1:1 kinetic binding model was used for data fitting (black curve) and values for on-rate (ka), off-rate (kd) and dissociation constant (KD) are given in the graph. c, ATPase activity of detergent-solubilized EfrCD in the presence and absence of 1 µM Nb_EfrCD#1. Shown is the mean and standard deviation of technical triplicates.
