Extended Data Fig. 7: Characterization of the interaction between FLOT1/2 and PipB2.
From: Photo-ANA enables profiling of host–bacteria protein interactions during infection
a, Western blot analysis (representative images from n = 2 independent experiments) of HA-tag pull-down experiment in HEK293T cells expressing full-length (FL) or LFNEF-deleted (Δ341-345) PipB2-HA. b, Fluorescence images (representative images from n = 2 independent experiments) of HeLa cells expressing FLOT1/2-GFP and PipB2-FLAG. PipB2 was visualized by anti-FLAG (red) antibody. DNA were detected by DAPI (blue). Rectangle indicates regions with magnified views shown in bottom right. Scale bar indicates 10 μm (5 μm for magnified views). c, Fluorescence images (representative images from n = 2 independent experiments) of S. Typhimurium (PipB2-STF)-infected HeLa cells expressing GFP-tagged FLOT2 at 20 hpi. PipB2 was visualized by anti-FLAG (red) antibody. Cell nuclei were stained with DAPI (blue). Bottom panel displays the magnified view of SCV region as indicated with rectangle. Scale bar indicates 5 μm.
