Extended Data Fig. 9: 7D12-32pcY-109Bpa forms a covalent bond with EGFR upon irradiation with 365 nm light.
From: Site-specific encoding of photoactivity and photoreactivity into antibody fragments
(A) In the Coomassie stained gel image, the band higher than EGFR is photocrosslinked 7D12 + EGFR complex. This is the full gel image for data shown in Fig. 5c. Lane marked L is the Invitrogen SeeBlue Plus2 Pre-stained Protein Standard (Catalog no. LC5925). These results demonstrate that 7D12-32pcY-109Bpa and 7D12-109Bpa form covalent bond with EGFR upon irradiation with 365 nm light. No crosslinked product was observed for wt-7D12 or 7D12-32pcY. These experiments were repeated twice with similar results. (B) Percentage crosslinked product calculated by measuring the intensity of the band corresponding to 7D12 + EGFR and EGFR.
