Fig. 1: New fluorinated Fz analog with improved brain performance.
From: An optimized bioluminescent substrate for non-invasive imaging in the brain
a, Structures of established NanoLuc substrates. b, Comparison between NanoLuc substrates in mice doubly hemizygous for Vgat-IRES-cre and CAG-LSL-Antares transgenes; top, linear pseudocolored; bottom, raw grayscale formats. c–f, Comparison between Fz and its fluorinated analogs in mice doubly hemizygous for Vgat-IRES-cre and CAG-LSL-Antares transgenes. Substrates were administered i.p. in a PEG-300-based solution. c, Chemical structures of Fz and its fluorinated analogs. d, Representative images with peak bioluminescence. e, Bioluminescence intensity in the brain over time. Data are presented as mean values ± s.e.m.; n = 4 animals for each compound or 3 animals for Fz. f, Statistical analysis of peak signal intensities from e. P values were determined by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc tests. Data are presented as mean values ± s.e.m.
