Extended Data Fig. 9: Effects of stimulation on bioluminescence from constitutive luciferases in awake mice.
From: An optimized bioluminescent substrate for non-invasive imaging in the brain
a. Example of data processing pipeline for the stimulation and imaging on head-fixed awake mice. b, Stimulation-dependent brain bioluminescence responses of mice doubly hemizygous for a CaMKIIα-cre driver and CAG-LSL-Antares reporter. c, Stimulation-dependent brain bioluminescence responses of mice doubly hemizygous for a CaMKIIα-cre driver and CAG-LSL-AkaLuc reporter. For b and c: Left, representative frames of an image sequence averaged and normalized from repeated 20-s periods of BLI; right, averaged and normalized cortical bioluminescence. Traces in a–c are averages of 57 stimulation cycles.
