Extended Data Fig. 4: Compound 13 improves brightness.
From: An optimized bioluminescent substrate for non-invasive imaging in the brain
Reporter mice were injected i.p. with 1.3 µmol substrate with 10 mg P-407. a, Comparison between 6 and Fz analogues with fluorination on the 2-position of the C8 ring and different groups in place of the furan ring, in mice doubly hemizygous for CAG-LSL-Antares reporter and Vglut2-IRES-cre driver; n = 5 animals in each group. b, Comparison between 6 and 13, in mice doubly hemizygous for CAG-LSL-Antares reporter and CaMKIIα-cre driver; n = 3 animals in each group. In a and b: Left, representative images with peak bioluminescence; right, bioluminescence intensity in the brain over time; data are presented as mean values ± SEM. c, Comparison of peak signal intensities for the results in b. Compound 13 was brighter, with P = 0.0033 by two-tailed Student’s unpaired t-test.
