Extended Data Fig. 6: Correlations between GPMV and BCR partitioning of membrane anchors before and after BCR clustering.

Points represent mean and s.e.m. of c(r < 50 nm) replotted from Extended data Fig. 3b (left) and c (right) versus means and s.e.m. of Lo enrichment from GPMV measurements replotted from Fig. 2b. Cell numbers for each condition are shown in Supplementary Fig. 3. trPAG* values were obtained from experiments where BCR was clustered in the presence of 5 µM PP2. Trends are fit to a linear model and the significance is assessed with a P value of the two-sided hypothesis test against the hypothesis of no correlation (lower values indicate greater significance), and an f-statistic that reports how well the variance in the data is described by the linear fit (higher values indicate greater significance). Gray shaded regions indicate the 95% confidence interval of the linear fit.