Extended Data Fig. 10: Summary of fit parameters describing anchor mobility at τ = 15 ms.

Diffusion coefficients (D) for the mobile component, the fraction of molecules in the confined state (α), and the confinement radius averaged over cells expressing the specified anchor for all conditions investigated. In all panels, vertical positions are means and s.e.m. of the specified parameter over multiple cells, with cell numbers for each anchor reported in Supplementary Fig. 3. Horizontal positions for all panels are means and s.e.m. replotted from Fig. 2b. Anchor labels ending in * indicate that values are from experiments where BCR was clustered in the presence of 5 µM PP2. For the condition ‘BCR proximal’, anchor localizations found within 100 nm of a BCR localization are cross-correlated with all anchor localizations as described in Methods. The fitting of BCR proximal curves was accomplished by fixing the confinement radius to the value obtained for all trajectories (τ = 2–10 min) to improve the robustness of fitting to correlation functions with reduced signal to noise. Trends in α and confinement radius are fit to a linear model and the significance is assessed with a P value of the two-sided hypothesis test against the hypothesis of no correlation (lower values indicate greater significance), and an f-statistic reports how well the variance in the data is described by the linear fit (higher values indicate greater significance). All points are included in the linear fit. Shaded regions indicate the 95% confidence interval of the fit.