Extended Data Fig. 3: Steady-state cross-correlation functions in cells before and after BCR crosslinking.

a, Average cross-correlation functions, c(r), between BCR and anchors in cells prior to BCR crosslinking (-Ag) or between 2 and 10 min after streptavidin addition (+Ag). Points represent the average over multiple cells and the shaded region represents the s.e.m.. Cell numbers interrogated for each anchor and distributions of c(r < 50 nm) are shown in Supplementary Fig. 3. b, c, Mean and s.e.m. for c(r < 50 nm) for cells imaged prior to BCR crosslinking (b) or between 2 and 10 min after BCR crosslinking (c) replotted from part a. All anchors show weak co-localization with unclustered BCR (c(r < 50 nm) close to 1.1). Amplitudes are more varied across anchors after BCR crosslinking. Anchor labels ending in * indicate that values are from experiments where BCR was clustered in the presence of 5 µM PP2. Distributions of c(r < 50 nm) values across anchors are presented in Supplementary Fig. 3.