Extended Data Fig. 6: NONO ligand (R)-SKBG-1 impairs cancer cell growth in a stereoselective and NONO C145-dependent manner.
From: Remodeling oncogenic transcriptomes by small molecules targeting NONO
a, Effects of (R)-SKBG-1 and (S)-SKBG-1 on the growth of sgControl and sgNONO MCF7 cells. b, Effects of (R)-SKBG-1 and (S)-SKBG-1 on the growth of sgNONO 22Rv1 cells expressing WT-NONO or C145S-NONO. c, Effects of (R)-SKBG-1 and (S)-SKBG-1 on the growth of HeLa and HT1080 cells. Cells were treated with DMSO or (R)-SKBG-1 or (S)-SKBG-1. After 6 days (22Rv1), 5 days (MCF7) or 4 days (HeLa and HT1080), cell growth was determined by CellTiterGlo, normalized to cells treated with only DMSO. Data are mean values ± s.e.m. normalized to DMSO-treated control cells for four independent replicates. EC50 values are reported with 95% confidence intervals shown in brackets. d, sgControl and sgNONO 22Rv1 cells show similar growth rates. 10,000 cells were seeded overnight, and then cell growth was measured by CellTiterGlo, normalized to day 0 signal. Data are mean values ± s.e.m. for four independent replicates.
