Extended Data Fig. 1: Molecular structures and fluorescent spectrums of FDAAs used in spatially resolved labeling of biofilm.
From: Spatial transcriptome uncovers rich coordination of metabolism in E. coli K12 biofilm
Molecular structures and fluorescent spectrums of FDAAs (Fluorescent D-alanine) used in spatially resolved labeling of biofilm. Shown in (a), (b) and (c) are molecular structures of HADA, NADA and TADA, respectively. The fluorescent spectrums of these three FDAAs are shown in (d); here dotted lines represent excitation spectrums and solid lines represent emission spectrums; the spectrum was measured by a microplate reader system (Tecan, Spark); FDAAs were at working concentration in M63B1 medium. (e) Labeling of FDAAs onto actively growing and dormant E. coli cells; here the seed culture was transferred to M63B1 medium with and without glucose to modulate the growth of the bacteria; and the FDAAs were added to stain the bacteria for three hours; shown is the merged image of phase contrast, CFP, GFP and mCherry channels under a 40× objective len; scale bar, 10 μm. Two biologically independent experiments were repeated with similar results.
