Extended Data Fig. 2: Perturbation of FDAAs and cell sorting on bacterial transcriptome.
From: Spatial transcriptome uncovers rich coordination of metabolism in E. coli K12 biofilm
Perturbation of FDAAs and cell sorting on bacterial transcriptome. (a) Workflow to test the impact of FDAAs on E. coli transcriptome. (b) Comparison of transcriptomes when treated with control (DMSO) or FDAAs at working concentrations; shown here is the consistency of transcriptomes in terms of expression (log2(TPM + 1)) correlation (n = 4,508; Pearson’s r = 0.97 for 10 µM HADA, Pearson’s r = 0.95 for 10 µM NADA, Pearson’s r = 0.96 for 25 µM TADA, Pearson’s r = 0.97 for 25 µM CADA). (c) Workflow to test whether cell sorting perturbs E. coli transcriptome. (d) Agreement between control (non-sorting) and sorting group, shown is the consistency of transcriptomes in terms of expression (log2(TPM + 1)) correlation (n = 4,508; Pearson’s r = 0.98).
