Extended Data Fig. 5: Evaluating changes in PPP and one-carbon metabolism.
From: Cytosolic and mitochondrial NADPH fluxes are independently regulated
a, Relative lactate enrichment in cells labeled with 1,2-13C2 glucose for 12 hours. b, Relative glucose uptake as measured by the depletion of glucose from media after culturing cells for 12 hours. c, Enrichment of AMP in cells labeled with 2,3,3-2H3 serine for 48 hours. Values are mean ± s.d; n = 6 biologically independent samples (WT in a and b, c) and n = 3 biologically independent samples (mIDH1 and mIDH2 in a and b). WT, HCT116 wild-type cells; mIDH1, mutant IDH1 cells; mIDH2, mutant IDH2 cells; AMP, adenosine monophosphate. Statistically significant differences were calculated by using a one-way ANOVA followed by Dunnett’s multiple comparison test (a-b). NS= no significant difference.
