Fig. 2: Proteoform detection based on peptide melting curve similarity.
From: Deep thermal profiling for detection of functional proteoform groups
a, Schematic of the method. b, Schematic of the domains of the TMPO alpha and beta isoforms. c, Melting curves of the median TMPO peptide signal (left) and by proteoforms as detected by the above outlined approach. d, Western blot validation (n = 1) of the detected proteoforms for TMPO (alpha on top, beta below) with differential thermal stability in the COG-355 cell line. e, Mapping of peptides assigned to the different proteoforms to the alpha and beta isoforms of TMPO.
