Extended Data Fig. 5: Functionally critical contacts of Dro with the ribosome as revealed by mutational analysis.
From: Inhibition of translation termination by the antimicrobial peptide Drosocin
The central image depicts the placement of glycosylated Dro in the NPET of the E. coli ribosome50. Functionally critical Dro residues are indicated in salmon; residues that tolerate multiple substitutions are marked in green. a-e, Functionally critical contacts involving Dro residues: a, Arg18, b, Arg15 and Pro16, c, Arg9, d, Arg4, e, Lys2. Panel f shows the ribosomal contacts of the Pro5-Pro8 segment of Dro where most amino acids substitutions do not impair the PrAMP’s activity.
