Extended Data Fig. 1: Dro acts upon translation termination but causes weak translation arrest at UGA stop codons.
From: Inhibition of translation termination by the antimicrobial peptide Drosocin
a, Inhibition of in vitro GFP translation by synthetic PrAMPs: class-I Oncocin112 (Onc112), class-II Api137, or non-glycosylated Dro (Dro). Bar graphs represent the normalized values of GFP fluorescence from reactions where RF1 was depleted (light grey) or supplemented (dark grey), setting the fluorescence value from reactions with or without RF1 in the absence of PrAMP as 100%. The error bars show standard deviation from the mean in three independent experiments. Significance levels indicated as NS, not significant; *, p-value <0.05; **, p-value < 0.01; ***, value < 0.001 (One-way ANOVA with Tukey’s Multiple Comparison test by GraphPad Prism). b, In vitro toeprinting analysis of the Api137 or Dro-mediated ribosome arrest at the UGA stop codon (red arrowhead) of the model yrbA ORF. The control reaction with no added PrAMPs is labeled as ‘none’. The control antibiotic retapamulin (Ret) stalls ribosomes at the start codon (green arrowhead). Sequencing reactions are labeled as C, U, A, G.
