Extended Data Fig. 7: Measurement of response curves of the regulatory systems and parameter fitting.
From: Unbalanced response to growth variations reshapes the cell fate decision landscape
(a) The expression level of this transcriptional repressor LacI is indicated via the intensity of fluorescent protein, mVenus, which is independently driven by the identical input promoter, that is, Psali. The output of this regulatory system is indicated by the same reporter. By this means, the input-output response function could quantitatively describe the properties of the interaction between the lac repressor and its binding site. The plasmids used are given in Supplementary Table 2. (b) Experimental measurements, and parameter fitting of the response curves for different circuits. For each of the measurements, various concentrations of sodium salicylate were used to induce the Psali promoter activity. Input and output were both identified as fluorescent intensity per mass using flow cytometry (see Methods). Only a moderate range of data (within the dotted box in the left panel) was taken for parameter fitting. The Hill coefficient n was fixed at 1.15 and other parameters were obtained using best-fit.
