Extended Data Fig. 10: Engineering metal-sensing strains of P. mirabilis.
From: Engineered bacterial swarm patterns as spatial records of environmental inputs
a. Maximum fold change of GFP, expressed from either pCopA or pCadA, achieved over the course of 17 hours at the indicated concentration normalized to uninduced wells, in plate reader. b. GFP fluorescence at 17 hours (end of experiment) for each concentration of each metal by strain. GFP fluorescence was calculated by subtracting background fluorescence value and dividing the raw fluorescence value by the media-background-subtracted OD600 value for the same well. In (a) and (b) dots represent individual wells. c. Colony radius of the copper-induced side of the plates, as shown in Fig. 4k. d. Lefthand plot: mean of the middle ring widths (that is, neither innermost nor outermost) of the colonies on the sides with copper. Each open circle represents a separate plate. Righthand plot: the same measurements normalized to the same day gfp strain’s measured mean widths at the given concentration. (c) and (d) show alternative representations of the data plotted in Fig. 4l; as in that figure, n = 9, 9, 6, and 9 plates for each of the two strains at 0, 10, 25, and 50 mM copper, respectively. Data are presented as mean values +/− standard deviation.
