Extended Data Fig. 7: Quality of the 70S–MAAAPQKC–TcmX reconstruction.
From: Tetracenomycin X sequesters peptidyl-tRNA during translation of QK motifs
a, Cryo-EM density map obtained for a 3D reconstruction of the 70S–MAAAPQKC–TcmX complex in Relion v4.0 (ref. 41) and auto-sharpened with Phenix v1.20.1 (ref. 45), filtered and colored by local resolution estimation values in Chimera X v.1.3 (ref. 49). b, Cross-section of the same map showing the E, P and A sites of the ribosome. c, Isolated ligand densities in the E, P and A sites, filtered and colored by local resolution. d, The same isolated densities shown in c, displayed as a transparent surface and fitted with molecular models of the E-, P- and A-site tRNAs, mRNA, TcmX and the nascent peptide. e, Model to map correlation curve calculated for the 70S–MAAAPQKC–TcmX structure. f, Representative cryo-EM densities of three different tRNA modifications within the A- and P-site tRNAs.
