Extended Data Fig. 6: Enzyme activity of FUT9 employing GDP-Fuc or GDP-CF3-Fuc donors and LNnT as acceptor.
From: Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9
FUT9 enzyme activity was determined as indicated in the presence or absence of GDP-Fuc or GDP-CF3-Fuc as donors and in the presence or absence of LNnT as acceptor. The reaction was monitored using the GDP-Glo assay format. The increase in signal over the no-enzyme blank reflects donor hydrolysis (in the absence of acceptor) or sugar transfer (in the presence of LNnT as acceptor). Plots show the mean values (bar) +/− s.d. (error bars) for n = 3 technical replicates (red circles).
