Extended Data Fig. 7: Rational search for target mutation sites through sequence alignment.
From: Engineered bacterial orthogonal DNA replication system for continuous evolution
Functional domains common to B-family DNAPs are shown above. All red and yellow shaded areas indicate regions that have been reported to affect φ29 DNAP fidelity. For example, mutations corresponding to the error-prone synthetic DNA of φ29 DNAP (shown in grey) through sequence alignment and homology analysis were found in GIL16 DNAP (shown in green).
