Extended Data Fig. 2: Purification and cryo-EM data imaging and processing of active, Gs-coupled AMY3R bound to San385.
From: Structural insight into selectivity of amylin and calcitonin receptor agonists
Top left, exemplar cryo-EM 2D class averages. Top middle, local resolution-filtered EM maps (consensus; Å). Top right, Fourier shell correlation (FSC) curves for the final consensus maps and half map validation, showing the overall nominal resolution. Bottom left, SEC trace of post FLAG-affinity column elution. The main peak was isolated and analysed by SDS-PAGE (insert). Bottom middle, local resolution-filtered EM maps (ECD focused refinement) displaying local resolution (Å). Bottom right, FSC curves for the focused refinement maps. Data are from an individual biochemical preparation.
