Extended Data Fig. 3: Purification and cryo-EM data imaging and processing of active, Gs-coupled complexes bound to San45.
From: Structural insight into selectivity of amylin and calcitonin receptor agonists
(A) San45-AMY3R complex. (B) San45-CTR complex. Within each panel: Top left, exemplar 2D cryo-EM class averages. Top middle, local resolution-filtered EM maps (consensus; Å). Top right, Fourier shell correlation (FSC) curves for the final consensus maps and map validation from half maps, showing the overall nominal resolution. Bottom left, SEC trace of post FLAG-affinity column elution. The main peak was isolated and analysed by SDS-PAGE (insert). Bottom middle, local resolution-filtered EM maps (receptor focused refinements) displaying local resolution (Å). Bottom right, FSC curves for the focused refinement maps. Data are from individual biochemical preparations.
